PS cells, a recently described mammary epithelial cell line, were cultured as previously described [26 (link)] in Advanced DMEM/HF-12 medium (Gibco) containing 4 ng/mL of hydrocortisone (Gibco), 2 mM of glutamine (Gibco) and 20 mM of HEPES (Biowhittaker), IGF-I (Insulin-like Growth Factor; 10 ng/mL; Peprotech), FGF (Fibroblast Growth Factor; 5 ng/mL; Peprotech) and EGF (Epidermal Growth Factor; 5 ng/mL; Sigma).
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