For reverse western blot, a human cytokine antibody array kits (R&D Systems) were used, according to the manufacturer's instructions. The western blotting analysis was performed with anti-STAT3, anti-Phospho STAT3, anti-CXCR2 (Cell Signaling, Danvers, MA), and anti-Actin antibody (Sigma, St. Louis, MO) as previously described [46 (link)].
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