Quantitative Collagen Imaging in Tissues
Corresponding Organization : Ospedale Policlinico San Martino
Variable analysis
- Picrosirius red staining
- Collagen expression (quantified under brightfield illumination)
- Image analysis performed using the Leica Q500 MC Image Analysis System (Leica, Cambridge, UK)
- A total of 10 fields randomly chosen per mouse
- Images viewed with brightfield illumination as well as with polarization contrast illumination at 40x
- No positive or negative controls were explicitly mentioned in the input protocol.
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