For GC-TOF-MS analysis, the redissolved samples were dried using a speed vacuum concentrator. The dried samples were derivatized as follows: (1) the oximation was performed by adding 50 µL of 20 mg/mL methoxyamine hydrochloride in pyridine to the dried samples and incubating at 30 °C for 90 min (2) the silylation was performed by mixing 50 µL of MSTFA to the mixture and incubating at 37 °C for 30 min. The derivatized samples were filtered using Millex GP 0.22 µm filters (Merck Millipore, Billerica, MA, USA).
GC-TOF-MS analysis was performed using an Agilent 7890A GC system (Santa Clara, CA, USA) equipped with Rtx-5MS (length 30 m, inner diameter 0.25 mm, J&W Scientific, Folsom, CA, USA) coupled with an Agilent 7693 autosampler and a Pegasus® HT TOF-MS (LECO Corp., St. Joseph, MI, USA). The analysis parameters were adapted from a previous study [2 (link)]. Three biological replications were analyzed for each sample.
GC-TOF-MS analysis was performed using an Agilent 7890A GC system (Santa Clara, CA, USA) equipped with Rtx-5MS (length 30 m, inner diameter 0.25 mm, J&W Scientific, Folsom, CA, USA) coupled with an Agilent 7693 autosampler and a Pegasus® HT TOF-MS (LECO Corp., St. Joseph, MI, USA). The analysis parameters were adapted from a previous study [2 (link)]. Three biological replications were analyzed for each sample.
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