Preparation of Cytotoxic Compounds

3-Methylcholanthrene (3MC) and CH223191 (1-Methyl-N-[2-methyl-4-[2-(2-methylphenyl)diazenyl]phenyl-1H-pyrazole-5-carboxamide) were purchased from Sigma-Aldrich (Milan, Italy). (3aS,4R,9bR)-4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-3H-cyclopenta [c] quinolone (G15) and 1-[2,(3,5-dimethoxyphenyl) ethenyl]-2,4-dimethoxybenzene (TMS) were obtained from Tocris Bioscience (Space, Milan, Italy). Mithramycin A (MTM A) was purchased from Abcam (Euroclone, Milan, Italy). CH223191 and G15 were dissolved in dimethyl sulfoxide (DMSO), 3MC in toluene and MTM A in ethanol. All reagents were used at concentrations previously reported [61 (link)–63 (link)].

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Cirillo F., Lappano R., Bruno L., Rizzuti B., Grande F., Guzzi R., Briguori S., Miglietta A.M., Nakajima M., Di Martino M.T, & Maggiolini M. (2019). AHR and GPER mediate the stimulatory effects induced by 3-methylcholanthrene in breast cancer cells and cancer-associated fibroblasts (CAFs). Journal of Experimental & Clinical Cancer Research : CR, 38, 335.

Publication 2019

3-Methylcholanthrene (3MC) CH223191

3 methylcholanthrene Ch223191 Dimethyl sulfoxide Ethanol Mithramycin Pyrazole 5 carboxamide Quinolone Toluene

Corresponding Organization : Magna Graecia University

Other organizations : University of Calabria, Istituto di Nanotecnologia, Azienda Ospedaliera di Cosenza, Life Science Institute, Kanazawa University

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Variable analysis

independent variables

3-Methylcholanthrene (3MC)
CH223191 (1-Methyl-N-[2-methyl-4-[2-(2-methylphenyl)diazenyl]phenyl-1H-pyrazole-5-carboxamide)
(3aS,4R,9bR)-4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-3H-cyclopenta [c] quinolone (G15)
1-[2,(3,5-dimethoxyphenyl) ethenyl]-2,4-dimethoxybenzene (TMS)
Mithramycin A (MTM A)

dependent variables

Not explicitly mentioned

control variables

Concentrations of the reagents were kept constant at previously reported levels [61–63]

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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