Exosomal Delivery of JNK for Targeted Therapy

Example 12

The binding of CIBN and CRY2 in cells expressing CIBN-EGFP-CD9 and JNK-mCherry-Cry2 at 488 nm wavelength blue light, and the loading of JNK within the exosome is evaluated.

For the massive production of JNK-loaded exosomes, cells stably expressing CIBN-EGFP-CD9 gene and JNK-mCherry-CRY2 gene are established, and exosomes are isolated and purified by Tangential Flow Filtration (TFF) method from culture supernatant.

Functional analysis of JNK-loaded exosomes is performed in target cells:

Target cells are treated with the JNK-loaded exosomes to show the functional activity.

Animal models are administered with the JNK-loaded exosomes by i.p. or i.v. to show therapeutic effect.