In the present study, the harvested ADSCs were
rinsed with PBS, transferred into 24-well plates
(7×104 cells/well), treated with e-CSF (E17, E18,
and E19) (10% v/v), and β-ME (10 ng/ml, Sigma
Aldrich, USA) for six days. The wells without any
treatment were considered as controls. Cell survival
and viability were measured by the MTT assay. MTT
(3-(4 (link),5 (link)-Dimethylthiazol-2 thiazolyl)-2,5- diphenyl 2
tetrazolium bromide) is a yellow tetrazolium dye that
responds to activated mitochondrial dehydrogenases
and changes the yellow color of samples to dark blue
formazan crystals. The cells were incubated with MTT
(5 mg/mL in PBS, Merck, Germany) at 37˚C for 3
hours. Finally, the absorbance was recorded at 570 nm
using a plate reader (ELx808TM, BioTek® instruments,
USA). Each experiment was repeated in triplicate (16 (link)).
rinsed with PBS, transferred into 24-well plates
(7×104 cells/well), treated with e-CSF (E17, E18,
and E19) (10% v/v), and β-ME (10 ng/ml, Sigma
Aldrich, USA) for six days. The wells without any
treatment were considered as controls. Cell survival
and viability were measured by the MTT assay. MTT
(3-(4 (link),5 (link)-Dimethylthiazol-2 thiazolyl)-2,5- diphenyl 2
tetrazolium bromide) is a yellow tetrazolium dye that
responds to activated mitochondrial dehydrogenases
and changes the yellow color of samples to dark blue
formazan crystals. The cells were incubated with MTT
(5 mg/mL in PBS, Merck, Germany) at 37˚C for 3
hours. Finally, the absorbance was recorded at 570 nm
using a plate reader (ELx808TM, BioTek® instruments,
USA). Each experiment was repeated in triplicate (16 (link)).
Free full text: Click here
