Multiplatform Lipidomic Analysis of PL-EVs

PL-EVs samples were analysed by a multiplatform using LC-MS and GC-MS. For LC-MS, an Agilent HPLC system (1290 infinity II series) coupled with a quadrupole time-of-flight analyser system (Q-ToF MS 6545) (Agilent Technologies, Santa Clara, CA, USA) was used. Additionally, for GC-MS, an Agilent GC system (1890 series) equipped with an autosampler MultiPurpose Sampler (MPS, Gerstel, Germany) coupled with a single quadrupole gas chromatography/mass spectrometry system (GC MSD 5977B series) was employed. Both techniques followed previously described methodologies [44 ,45 (link),46 (link)]. Full descriptions of QC preparation, detailed instrumental description, data treatment, metabolite annotation, and statistical analysis are available in the Supplementary Data.