We used brain tissues from humans, HIP, WT and AKO rats. Tissues were processed as previously described6 (link),29 (link). Antibodies against amylin (1:200, T-4157, Bachem-Peninsula Laboratories, CA), glial fibrillary acidic protein (GFAP; 1:600, MAB360, Millipore, MA) or myelin basic protein (MBP; 1:5,000, AMAB91064, clone CL2829, Sigma, MO) were the primary antibodies. Anti-mouse or anti-rabbit IgG (1:50, A3562, A3687, Sigma, MO) were secondary antibodies. The specificity of the amylin antibody in both human and rat brain tissues was established in previous studies6 (link),7 (link),29 (link).
Protocol detail
Immunohistochemistry of Amylin, GFAP, and MBP
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Amylin
Anti igg
Antibodies
Antibody specificity
Brain
Clone
Gfap
Human
Mouse
Myelin basic protein
Rabbit
Tissues
Corresponding Organization : University of Kentucky
Other organizations : United States Department of Veterans Affairs, University Medical Center Utrecht
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Variable analysis
independent variables
- Brain tissues from humans, HIP, WT and AKO rats
- Amylin expression
- Glial fibrillary acidic protein (GFAP) expression
- Myelin basic protein (MBP) expression
- Tissue processing as previously described in references 6 and 29
- The specificity of the amylin antibody in both human and rat brain tissues was established in previous studies (references 6, 7, and 29)
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