Comprehensive Metabolic Profiling and Insulin Sensitivity Assessment

Blood and urine samples were taken after 12 h of fasting. Plasma renin activity (GammaCoat Plasma Renin Activity 125-I RIA Kit, DiaSorin, Saluggia, Italy) and aldosterone concentration (Active Aldosterone RIA, Beckman Coulter, Fullerton, CA, USA) were determined using commercial kits. Plasma C-reactive protein (CRP), sodium, potassium, glucose, creatinine, cystatin C, uric acid, triglyceride, and total, HDL and LDL (high- and low-density lipoprotein, respectively) cholesterol concentrations were determined using Cobas Integra 800 (F. Hoffmann-LaRoche Ltd, Basel, Switzerland). Insulin and parathyroid hormone (PTH) were determined using electrochemiluminescence immunoassay (Cobas e411, Roche Diagnostics). Estimated glomerular filtration rate (eGFR) was calculated using the Chronic Kidney Disease – Epidemiology collaboration (CKD-EPI) cystatin C equation^{27 (link)}. Insulin sensitivity was evaluated by the quantitative insulin sensitivity check index (QUICKI)^{28 (link)}, and homeostatic model assessment of insulin resistance (HOMA-IR)^{29 (link)}. In addition, the subjects were invited to participate in a standard 75-g oral glucose tolerance test (OGTT) for the determination of the Matsuda index^{30 (link)}.

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Kokko E., Nevalainen P.I., Choudhary M.K., Koskela J., Tikkakoski A., Huhtala H., Niemelä O., Viukari M., Mustonen J., Matikainen N, & Pörsti I. (2020). Aldosterone-to-renin ratio is related to arterial stiffness when the screening criteria of primary aldosteronism are not met. Scientific Reports, 10, 19804.

Publication 2020

GammaCoat Plasma Renin Activity 125-I RIA Kit Active Aldosterone RIA Cobas Integra 800 Cobas e411

Aldosterone Blood C reactive protein Cholesterol Creatinine Cystatin c Diagnostics Glomerular filtration rate Glucose Homeostatic Immunoassay Insulin Insulin resistance Insulin sensitivity Low density lipoprotein Oral glucose tolerance test Parathyroid hormone Plasma Potassium Renin Sodium Triglyceride Uric acid Urine

Corresponding Organization : Tampere University Hospital

Other organizations : Seinäjoki University of Applied Sciences, Helsinki University Hospital, University of Helsinki

Top 5 similar protocols

Variable analysis

independent variables

Fasting duration (12 h)

dependent variables

Plasma renin activity
Aldosterone concentration
Plasma C-reactive protein (CRP)
Sodium
Potassium
Glucose
Creatinine
Cystatin C
Uric acid
Triglyceride
Total cholesterol
HDL cholesterol
LDL cholesterol
Insulin
Parathyroid hormone (PTH)
Estimated glomerular filtration rate (eGFR)
Insulin sensitivity (QUICKI, HOMA-IR)
Matsuda index

control variables

Blood and urine samples were taken after 12 h of fasting

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