Fecal Microbiome Profiling in Mice

DNA was extracted from mice fecal samples using the Powerfood Microbial DNA Isolation kit (Mo Bio Laboratories, Inc., Carlsbad, CA, United States) according to the manufacturer’s protocol. Intestinal bacterial community compositions were analyzed using 16S rRNA gene sequencing, which was performed according to a previous report (Ryu et al., 2022 (link)). The obtained data were analyzed using Mothur (v. 1.45.3). To visualize differences in the microbiota composition between groups, nonmetric multidimensional scaling plots were generated on the basis of unweighted and weighted UniFrac distances. The α-diversity and relative abundance of OTUs at family and genus levels were analyzed using the MicrobiomeAnalyst web-based platform (Chong et al., 2020 (link)).