Measuring Anti-HIV-1 Env Antibody Titers

Serum IgG titers against HIV-1 C.1086 Env gp140 were determined by enzyme-linked immunosorbent assays (ELISAs) using standard protocols (21 (link)). Baseline sera from each animal served as negative control, and optical density (OD) values twofold above baseline were considered positive and extrapolated to determine anti-Env antibody concentrations. Antibody levels in rectal secretions were measured using a binding antibody multiplex assay using C.1086 gp140 K160N-labeled magnetic beads (MagPlex; Bio-Rad) as previously described (62 (link)). C.1086 gp120 (from B. Haynes) coated on the magnetic beads was used.

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Elizaldi S.R., Verma A., Walter K.A., Rolston M., Dinasarapu A.R., Durbin-Johnson B.P., Settles M., Kozlowski P.A., Raeman R, & Iyer S.S. (2019). Rectal Microbiome Composition Correlates with Humoral Immunity to HIV-1 in Vaccinated Rhesus Macaques. mSphere, 4(6), e00824-19.

Publication 2019

MagPlex

Animal Anti antibody Antibody Assay Enzyme linked immunosorbent assays Gp120 Gp140 Gp140 hiv 1 Rectal Secretions Sera Values

Corresponding Organization :

Other organizations : University of California, Davis, Louisiana State University Health Sciences Center New Orleans, Emory University, University of Pittsburgh

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum IgG titers against HIV-1 C.1086 Env gp140
Antibody levels in rectal secretions

control variables

Baseline sera from each animal served as negative control

controls

Baseline sera from each animal served as negative control
Optical density (OD) values twofold above baseline were considered positive

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