HPLC Analysis of Compounds

All HPLC tests were carried out on a Shimadzu Nexera (Shimadzu Corp., Kyoto, Japan) [23 (link)]. A Dr. Maisch ReproSil-Pur Basic-C18 100 column with 5 µm and 100 × 4.60 mm particle sizes (Dr. Maisch, Ammerbuch-Entringen, Germany) was utilized for the stationary phase. Methanol/0.1% formic acid (70:30 v/v) was used as the mobile phase. The mobile phase was vacuum-filtered via a 0.45 µm nylon filter. The experimental parameters were as follows: flow rate of 1.0 mL·min⁻¹, wavelength of 308 nm, and column temperature of 35 °C.

Free full text: Click here

Rosiak N., Tykarska E, & Cielecka-Piontek J. (2024). Enhanced Antioxidant and Neuroprotective Properties of Pterostilbene (Resveratrol Derivative) in Amorphous Solid Dispersions. International Journal of Molecular Sciences, 25(5), 2774.

Publication 2024

ReproSil-Pur Basic-C18 100 column

Corresponding Organization : Poznan University of Medical Sciences

Top 5 similar protocols

Variable analysis

independent variables

Type of HPLC system: Shimadzu Nexera

dependent variables

Detected compound(s) at 308 nm wavelength

control variables

Stationary phase: Dr. Maisch ReproSil-Pur Basic-C18 100 column with 5 µm and 100 × 4.60 mm particle sizes
Mobile phase: Methanol/0.1% formic acid (70:30 v/v), vacuum-filtered via a 0.45 µm nylon filter
Flow rate: 1.0 mL·min^-1
Wavelength: 308 nm
Column temperature: 35 °C

controls

No positive or negative controls were explicitly mentioned in the information provided.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!