P. gingivalis W83 was grown in hemin (5 µg/mL) (Solarbio, Beijing, China) and menadione (1 µg/mL) (Solarbio, Beijing, China) containing brain heart infusion broth (BHI) (Oxiod, Basingstoke, UK) anaerobically (37 °C, 85% N2, 10% H2, 5% CO2). 10^9 CFU/mL bacteria were collected for following use.
For mice oral infection, the bacterial precipitates obtained after centrifugation were resuspended in a 2% carboxymethylcellulose (CMC) solution.
In the case of the bacteria-cell co-culture experiments, the bacterial precipitates obtained after centrifugation were resuspended in 10% fetal bovine serum (FBS)-containing DMEM and then co-cultured with cells at a multiplicity of infection (MOI) of 100. [22 , 23 (link)].
For mice oral infection, the bacterial precipitates obtained after centrifugation were resuspended in a 2% carboxymethylcellulose (CMC) solution.
In the case of the bacteria-cell co-culture experiments, the bacterial precipitates obtained after centrifugation were resuspended in 10% fetal bovine serum (FBS)-containing DMEM and then co-cultured with cells at a multiplicity of infection (MOI) of 100. [22 , 23 (link)].
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