Cells were plated in a 96-well plate and treated with the indicated concentration of phenformin for 24 h. Using NAD+/NADH Glo Assay (Promega), the NAD+/NADH ratio was calculated [6 (link)].
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Protocol detail
Measuring NAD+/NADH Ratio in Cells
Corresponding Organization : Seoul National University
Other organizations : National Cancer Center, Ewha Womans University, Chungnam National University, Korea Institute of Toxicology
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Variable analysis
independent variables
- Phenformin concentration
- NAD+/NADH ratio
- Cells were plated in a 96-well plate
- Cells were treated for 24 h
- No positive or negative controls were explicitly mentioned in the provided information.
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