Mouse Transgenic Models for Immunology

Foxp3^YFP-Cre (Ref.³²), Foxp3^GFP-Cre-ERT2 (Ref.³³), Rosa26^{STOP f/f-YFP} (Ref.^{34 (link)}), Rosa26^{STOP f/f-IKK2ca} (Ref.^{35 (link)}), Ifng KO,³⁶ B6-scurfy (Ref.^{37 (link)}) and C57BL/6/J mice were purchased from Jackson laboratories. Ramnik J. Xavier and James J. Moon (MGH) provided CARMA1^f/f (Ref.^{38 (link)}) and Rag1 KO mice, respectively. Animals were housed in specific pathogen-free facilities at the Massachusetts General Hospital (MGH) and all experimental studies were approved and performed in accordance with guidelines and regulations implemented by the MGH Institutional Animal Care and Use Committee (IACUC). For survival studies the age of mice at euthanasia mandated by a moribund state of health was recorded in Kaplan Meyer plots.

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Pilato M.D., Kim E.Y., Cadilha B.L., Pruessmann J.N., Nasrallah M.N., Seruggia D., Usmani S.M., Misale S., Zappulli V., Carrizosa E., Mani V., Ligorio M., Warner R.D., Medoff B.D., Marangoni F., Villani A.C, & Mempel T.R. (2019). Targeting the CBM complex causes Treg cells to prime tumors for immune checkpoint therapy. Nature, 570(7759), 112-116.

Publication 2019

Foxp3YFP-Cre Foxp3GFP-Cre-ERT2 Rosa26STOP f/f-YFP Rosa26STOP f/f-IKK2ca C57BL/6/J

Animals C57bl mice Ert2 Euthanasia Ifng Institutional animal care and use committee Mice Rag1 Specific pathogen free

Corresponding Organization : Massachusetts General Hospital

Other organizations : Boston Children's Hospital, Center for Cancer Research, University of Padua

Top 5 similar protocols

Variable analysis

independent variables

Foxp3^YFP-Cre (Ref.32)
Foxp3^GFP-Cre-ERT2 (Ref.33)
Rosa26^STOP f/f-YFP (Ref.34)
Rosa26^STOP f/f-IKK2ca (Ref.35)
Ifng KO (Ref.36)
B6-scurfy (Ref.37)

dependent variables

Survival studies the age of mice at euthanasia mandated by a moribund state of health was recorded in Kaplan Meyer plots

control variables

C57BL/6/J mice
CARMA1^f/f (Ref.38)
Rag1 KO mice

positive controls

C57BL/6/J mice

negative controls

Rag1 KO mice

Annotations

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