Oral Glucose Tolerance Test Protocol

An oral glucose tolerance test (OGTT) was performed three days prior to initiating treatment with the AE and three days prior to euthanasia. The animals were submitted for 8 h of fasting [41 (link)]. The fasting glucose was verified via the flow rate (time 0) using a G-tech glucometer (G-Tech Free, Infopia Co., Ltd., Anyang, Gyeonggi-do city, Korea) with the tail blood. After this, the animals received D-glucose (Vetec, Duque de Caxias, RJ, Brazil) at 2 g/kg of body weight by oral gavage, and the blood glucose was monitored at 15, 30, 60, and 120 min after glucose administration via the tail blood. The area under the curve (AUC) was calculated for each animal, and the mean was calculated for each experimental group [41 (link),50 (link)].