Agilent LC-MS/MS method for tetrodotoxin

An Agilent 1290 Infinity LC system (Agilent Technologies Deutschland GmbH, Waldbronn, Germany) was used for the liquid chromatographic separation. This separation is described in Leão et al. [18 (link)]. The chromatographic conditions used in the analysis of TTX are summarized in Table S6 (Supplementary Materials).
A 6460A Triple Quadrupole mass/massMS/MS (QQQ) equipped with a Jet Stream ESI source (Agilent Technologies Deutschland GmbH Waldbronn, Germany) was used for the analysis of TTX in MRM (multiple reaction monitoring) mode by detecting m/z transitions in tandem mass spectrometry, which are included in Table S7 of Supplementary Materials [20 ].
The optimized conditions are summarized in Table S8 of Supplementary Materials [18 (link)].

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López Cabo M., Romalde J.L., Simal-Gandara J., Gago Martínez A., Giráldez Fernández J., Bernárdez Costas M., Pascual del Hierro S., Pousa Ortega Á., Manaia C.M., Abreu Silva J, & Rodríguez Herrera J. (2020). Identification of Emerging Hazards in Mussels by the Galician Emerging Food Safety Risks Network (RISEGAL). A First Approach. Foods, 9(11), 1641.

Publication 2020

Agilent 1290 Infinity LC system Jet Stream ESI source

Chromatographic Liquid chromatographic Tandem mass spectrometry

Corresponding Organization : Consejo Superior de Investigaciones Científicas

Other organizations : Universidade de Santiago de Compostela, Universidade de Vigo, Xunta de Galicia, Universidade Católica Portuguesa

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