Human SCNT Pluripotent Cell Lines

Human SCNT pluripotent cell lines, CHA-SCNT-hPSC-17 [10 (link)] and hPSC-18 ([10 (link)], Korea Stem Cell Registry code hES12019001), and embryonic stem cell line, CHA-hESC-15 ([25 (link)], Korea Stem Cell Registry code hES12010028), were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs) in DMEM/F12 medium supplemented with 20% KnockOut Serum Replacement (KO-SR), 1% non-essential amino acids (NEAA), 0.1 mM β-mercaptoethanol (β-ME), and 4 ng/mL hrbFGF (ES culture media; all of them from Invitrogen). Human PSCs were mechanically passaged every 5 days under a stereomicroscope (SMZ 645, Nikon, Tokyo, Japan).

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Jung S.K., Lee J.E., Lee C.W., Shim S.H, & Lee D.R. (2021). Rapid Production and Genetic Stability of Human Mesenchymal Progenitor Cells Derived from Human Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells. International Journal of Molecular Sciences, 22(17), 9238.

Publication 2021

hrbFGF SMZ 645

Cell lines Culture media Embryonic Embryonic stem cell Essential amino acids Fibroblasts Hesc Human Mercaptoethanol Mouse Pscs Serum Stem cell

Corresponding Organization :

Other organizations : CHA University, CHA Bundang Medical Center

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Variable analysis

independent variables

Cell lines: CHA-SCNT-hPSC-17, hPSC-18, and CHA-hESC-15

dependent variables

Not explicitly mentioned

control variables

Mitotically inactivated mouse embryonic fibroblasts (MEFs) as feeder cells
DMEM/F12 medium supplemented with 20% KnockOut Serum Replacement (KO-SR), 1% non-essential amino acids (NEAA), 0.1 mM β-mercaptoethanol (β-ME), and 4 ng/mL hrbFGF (ES culture media)
Mechanical passaging of human PSCs every 5 days under a stereomicroscope

controls

Positive control: Not specified
Negative control: Not specified

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