Antibodies for SFTSV Protein Detection

Rabbit antisera to SFTSV N, GP (GN), or RdRp were described previously [15 (link), 17 (link)]. Mouse anti-N serum was raised against SFTSV N protein prepared from Escherichia coli. Mouse anti-Flag (Sigma-Aldrich, Cat#F3165), anti-HA (Sigma-Aldrich, Cat#05–904) and anti-IFNAR1 (Sigma-Aldrich, Cat#SAB1406003) antibodies and rabbit anti-MOV10 (Proteintech, Cat#10370-1-AP), anti-β-actin (ABclonal, Cat#AC026), anti-Stag (Abcam, Cat#ab18588) and anti-GFP (Abcam, Cat#ab6556) antibodies were purchased from the indicated manufacturers. For the secondary antibodies, goat anti-mouse IgG conjugated with Alexa Fluor 488 (Thermo Fisher Scientific, Cat#A-11001) and goat anti-rabbit IgG conjugated with Alexa Fluor 647 (Thermo Fisher Scientific, Cat#A32733) were used in the IFA assay; goat anti-mouse or anti-rabbit IgG antibodies conjugated with HRP (Abcam, Cat#ab6789 and Cat#ab6721) were used for IB analysis.

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Mo Q., Xu Z., Deng F., Wang H, & Ning Y.J. (2020). Host restriction of emerging high-pathogenic bunyaviruses via MOV10 by targeting viral nucleoprotein and blocking ribonucleoprotein assembly. PLoS Pathogens, 16(12), e1009129.

Publication 2020

Mouse anti-Flag anti-HA anti-β-actin anti-Stag anti-GFP goat anti-mouse IgG conjugated with Alexa Fluor 488

Actin Alexa fluor 488 Alexa fluor 647 Anti igg Antibodies Antisera Assay Cat 1 Escherichia coli Goat Mouse N protein Rabbit Serum

Corresponding Organization : University of Chinese Academy of Sciences

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Variable analysis

independent variables

Rabbit antisera to SFTSV N, GP (GN), or RdRp
Mouse anti-N serum raised against SFTSV N protein prepared from Escherichia coli
Mouse anti-Flag, anti-HA, and anti-IFNAR1 antibodies
Rabbit anti-MOV10, anti-β-actin, anti-Stag, and anti-GFP antibodies
Goat anti-mouse IgG conjugated with Alexa Fluor 488
Goat anti-rabbit IgG conjugated with Alexa Fluor 647
Goat anti-mouse or anti-rabbit IgG antibodies conjugated with HRP

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Rabbit antisera to SFTSV N, GP (GN), or RdRp

negative controls

Not explicitly mentioned

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