Anti-Mcm7 (yN-19, sc-6688, Santa Cruz), anti-Orc6 (SB49). Psf2- FLAG was visualized with ANTI-FLAG M2-peroxidase (Sigma), Mcm10 was detected using the T7-tag antibody (69522, Novagen), Dbf4 was visualized with an anti-CBP tag antibody (07-482 Merck Millipore) and Rpa1 was detected with anti-scRPA (AS07 214, Agrisera). Psf1 antibodies were a gift from K. Labib. Polyclonal antibodies against Sld3, Sld7, Sld2, Cdc45 and Dpb11 were described previously6 (link). Polyclonal antibodies against the Dpb2 subunit of pol ε were raised against full-length protein, and their specificity was confirmed using purified pol ε.
Protocol detail
Immunoblotting of Eukaryotic DNA Replication Proteins
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Other organizations : Cancer Research UK
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Variable analysis
independent variables
- Anti-Mcm7 (yN-19, sc-6688, Santa Cruz)
- Anti-Orc6 (SB49)
- Psf2-FLAG was visualized with ANTI-FLAG M2-peroxidase (Sigma)
- Mcm10 was detected using the T7-tag antibody (69522, Novagen)
- Dbf4 was visualized with an anti-CBP tag antibody (07-482 Merck Millipore)
- Rpa1 was detected with anti-scRPA (AS07 214, Agrisera)
- Psf1 antibodies were a gift from K. Labib
- Polyclonal antibodies against Sld3, Sld7, Sld2, Cdc45 and Dpb11 were described previously
- Polyclonal antibodies against the Dpb2 subunit of pol ε were raised against full-length protein, and their specificity was confirmed using purified pol ε
- Not explicitly mentioned
- Not explicitly mentioned
- Positive controls: Not specified
- Negative controls: Not specified
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