Integrin-mediated signaling regulation

Antibodies for α4-integrin immunoprecipitation (PS/2 and HP2/1) and blotting (C-20) were from Millipore, GeneTex, and Santa Cruz Biotechnology, respectively. The phospho-α4-integrin antibody was generated as previously described (22 (link)). PKA subunit antibodies were supplied by Becton Dickinson. An anti–VCAM-1 antibody was from Southern Biotech. H89 was obtained from Millipore, and 8-bromoadenosine cAMP (8-Br-cAMP) was from Enzo Life Sciences. The PRKAR1β cDNA was obtained from GeneCopoeia. Streptozotocin (STZ) and anti-actin and control antibodies were from Sigma-Aldrich.

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Abplanalp W.T., Conklin D.J., Cantor J.M., Ginsberg M.H., Wysoczynski M., Bhatnagar A, & O’Toole T.E. (2016). Enhanced Integrin α4β1–Mediated Adhesion Contributes to a Mobilization Defect of Endothelial Progenitor Cells in Diabetes. Diabetes, 65(11), 3505-3515.

Publication 2016

anti–VCAM-1 antibody Streptozotocin (STZ)

8 bromoadenosine Actin Anti antibody Antibodies Antibody Cdna Immunoprecipitation Integrin Streptozotocin Subunit Vcam 1

Corresponding Organization : University of Louisville

Other organizations : University of California, San Diego

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Variable analysis

independent variables

Streptozotocin (STZ)
8-bromoadenosine cAMP (8-Br-cAMP)
PRKAR1β cDNA

dependent variables

Phospho-α4-integrin
VCAM-1

control variables

Anti-actin
Control antibodies

controls

Phospho-α4-integrin antibody generated as previously described (22)
Control antibodies

Annotations

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