Cigarette smoke extraction (CSE) was prepared as previously described [15 (link)]. Antibodies against β-actin (anti-β-actin, 66009-1-Ig), TXNRD1 (anti-TXNRD1, 11117-1-AP), and Nrf2 (anti-Nrf2, 16396-1-ap) were purchased from Proteintech. HO-1 (anti-HO-1, GB11845) was purchased from Servicebio. Auranofin (Ridaura, SKF-39162), the inhibitor of thioredoxin reductase (TXNRD1), was purchased from Selleck.cn.
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