Histopathological Analysis of Tissues Post Euthanasia

Immediately after euthanasia, macroscopic changes were recorded, and the brain, heart, lungs, liver, spleen, and right kidneys were immersed in buffered 10% formalin, processed for paraffin embedding, sectioned at 5 μm, and stained with haematoxylin and eosin (H&E). Grocott's modification of Gomori's methenamine-silver (GMS) stain was used for detection of fungi in situ [35 (link), 37 (link)]. Histopathology images from sections of formalin-fixed and paraffin-embedded tissues stained with GMS or H&E were obtained using a light microscope (Olympus BX51) fitted with a digital camera (Nikon DXM1200F) and ScanScope XT digital scanner (Aperio). Images were processed with Adobe Photoshop and Aperio ImageScope v11.1.2.760 (Aperio).

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Navarathna D.H., Pathirana R.U., Lionakis M.S., Nickerson K.W, & Roberts D.D. (2016). Candida albicans ISW2 Regulates Chlamydospore Suspensor Cell Formation and Virulence In Vivo in a Mouse Model of Disseminated Candidiasis. PLoS ONE, 11(10), e0164449.

Publication 2016

Nikon DXM1200F

Brain Eosin Euthanasia Formalin Fungi Haematoxylin Heart Kidneys Light microscope Liver Lungs Paraffin Scanner Spleen Tissues

Corresponding Organization : National Institute of Allergy and Infectious Diseases

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Macroscopic changes
Histopathology of the brain, heart, lungs, liver, spleen, and right kidneys

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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