In this section we show the analysis of inflammasome activation by FlowSight, performed utilizing ASC speck formation in LPS+Nig stimulated-THP1derived macrophage.
Inflammasome Activation Analysis by Flow Cytometry
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Corresponding Organization : Leibniz Association
Other organizations : Friedrich-Alexander-Universität Erlangen-Nürnberg, Kiel University, Technical University of Denmark, Lund University, Stanford Medicine, Stanford University, University of Zurich, Inserm, Université Paris Cité, Assistance Publique – Hôpitaux de Paris, Hôpital Saint-Louis, University of Lübeck, Humanitas University, University of Birmingham, Technical University of Munich, University of Florence, University of Siena, National Cancer Centre Singapore, Duke-NUS Medical School, Sapienza University of Rome, Italian Institute of Technology, Istituto Pasteur, Medizinische Hochschule Hannover, Istituti di Ricovero e Cura a Carattere Scientifico, Fondazione Santa Lucia, Medical University of Vienna, University of California, Davis, Ludwig-Maximilians-Universität München, Urologische Klinik München, European Molecular Biology Laboratory, University of Technology Sydney, Helmholtz Zentrum München, Ghent University, VIB-UGent Center for Inflammation Research, University College Dublin, University of British Columbia, British Columbia Children's Hospital, Universitätsklinikum Erlangen, Monash University, Albert Einstein College of Medicine, California Institute for Regenerative Medicine, Thermo Fisher Scientific (United States), Luxembourg Institute of Health, University of Southern Denmark, Odense University Hospital, University of Luxembourg, Terry Fox Research Institute, University of Auckland, Maurice Wilkins Centre, German Center for Infection Research, Institute for Advanced Study, Miltenyi Biotec (Germany), University of Rochester Medical Center, University of Verona, Peter Doherty Institute, University of Melbourne, Nankai University, University of Gothenburg, FIRC Institute of Molecular Oncology, Universitat de Barcelona, Délégation Paris 5, NYU Langone Health, Princess Margaret Cancer Centre, King's College London, National Health Service, Helmholtz Centre for Infection Research, Don Carlo Gnocchi Foundation, University of Milan, University of Milano-Bicocca, University of Cambridge, University of Leicester, de Duve Institute, Institut Pasteur, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Australian Regenerative Medicine Institute, Australian Research Council, The Francis Crick Institute, Istituto Giannina Gaslini, Novo Nordisk Foundation, University of Copenhagen, University Hospital Regensburg, University of Regensburg, Vita-Salute San Raffaele University, Istanbul University, Sony (United Kingdom), Charité - Universitätsmedizin Berlin, University of Palermo, University of Bonn, Agency for Science, Technology and Research, Singapore Immunology Network, University of Oxford, Freie Universität Berlin, Humboldt-Universität zu Berlin, University of Toronto, Heidelberg Institute for Stem Cell Technology and Experimental Medicine, University of Bern, Eötvös Loránd University, Leibniz Institute of Environmental Medicine, Leiden University Medical Center, Washington University in St. Louis, Centro de Investigacion Principe Felipe, Newcastle University, Institut Necker Enfants Malades, Centre National de la Recherche Scientifique, Hôpital Necker-Enfants Malades, Sorbonne Paris Cité, University of Freiburg, University Medical Center Freiburg, Owl Biomedical (United States), Nottingham Trent University, Leibniz Institute of Photonic Technology, University of Arizona, Henan University, Oncode Institute, The Netherlands Cancer Institute, Fundação Oswaldo Cruz, University of Massachusetts Chan Medical School, Istituto Nazionale Genetica Molecolare, University of Veterinary Medicine Vienna, SingHealth Duke-NUS Academic Medical Centre, Shanghai Jiao Tong University, University of California, Los Angeles, The University of Tokyo, Wuhan University of Technology, University of Cologne, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, University of Glasgow, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, German Cancer Research Center, Heidelberg University, German Cancer Society, Karolinska University Hospital, Murdoch Children's Research Institute, Houston Methodist, Universitat de València, INCLIVA Health Research Institute, MRC Epidemiology Unit, University Hospital of Basel, University of Minnesota, Delft University of Technology, Karolinska Institutet, Amsterdam University Medical Centers, University of Amsterdam, Sanquin, New Mexico State University, University Hospital Schleswig-Holstein, Chinese Academy of Medical Sciences & Peking Union Medical College, Shenzhen University, Shenzhen Institutes of Advanced Technology, University of Pennsylvania, Singapore General Hospital, Max Planck Institute for Infection Biology, University of Rijeka, Charles University, Jena University Hospital, National University of Singapore, ETH Zurich, Tulane University, Medical University of Graz, Goethe University Frankfurt, Max Planck Institute for Biology of Ageing, Berlin-Brandenburger Centrum für Regenerative Therapien, Osaka University, Nanyang Technological University, Université Paris Sciences et Lettres, Institut Curie, University of Chieti-Pescara, Institut Cochin, Caprion (Canada), Philipps University of Marburg, Beckman Coulter Foundation, Pompeu Fabra University, The Ohio State University, Erasmus MC, Centre d’Immunologie de Marseille-Luminy, Aix-Marseille Université, Kantonsspital St. Gallen, Leibniz Institute of Virology (LIV), Roswell Park Comprehensive Cancer Center, Fred Hutch Cancer Center, IRCCS Humanitas Research Hospital, William Harvey Research Institute, University of Manitoba, National Institute of Geriatrics, Rheumatology and Rehabilitation, Institute for Experimental Endocrinology and Oncology, University of Sydney, Leipzig University, Northwestern University, Trinity College Dublin, Linköping University, Bambino Gesù Children's Hospital, Helmholtz Centre for Environmental Research, Klinikum rechts der Isar, Chiba University, University Medical Center Hamburg-Eppendorf, Universität Hamburg, Queen Mary University of London, University of Michigan–Ann Arbor, Centro de Investigación Biomédica en Red de Cáncer, Centro de Investigación del Cáncer, Instituto de Investigación Biomédica de Salamanca, Universidad de Salamanca, Burnet Institute, Amgen (United States), Universitätsmedizin Greifswald, Siriraj Hospital, Mahidol University, University of Tartu, Josep Carreras Leukaemia Research Institute, Universitat Autònoma de Barcelona, University of Udine, RMIT University, Utrecht University, University Medical Center Utrecht, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Max Delbrück Center, Purdue University West Lafayette, Groene Hart Ziekenhuis, University of Alberta, Ospedale Policlinico San Martino, RIKEN Center for Integrative Medical Sciences, University of Lausanne, Istituto Neurologico Mediterraneo, Centre de Recherche des Cordeliers, University Hospital Bonn, Otto-von-Guericke University Magdeburg, Translational Research Institute, University of Lisbon, University of Brescia, Ashland (United States), BD Biosciences (United States), National Institutes of Health, Fraunhofer Institute for Cell Therapy and Immunology, Tsinghua University, Novartis (Switzerland), Pennsylvania State University, Alexander Fleming Biomedical Sciences Research Center, Universidade de São Paulo, CK-CARE, University Medical Center of the Johannes Gutenberg University Mainz, Johannes Gutenberg University Mainz, Babraham Institute, The University of Texas MD Anderson Cancer Center, Westmead Institute for Medical Research, University College London, University of London, Friedrich Schiller University Jena, TU Dortmund University, Leibniz Research Centre for Working Environment and Human Factors, University of Göttingen, Universitätsmedizin Göttingen, Imperial College London, Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Simon Fraser University, Sichuan University, West China Hospital of Sichuan University, University Hospital of Bern
Protocol cited in 16 other protocols
Variable analysis
- LPS+Nig stimulation of THP1-derived macrophages
- ASC speck formation
- Caspase-1 activity
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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