Mapping Fusarium Resistance Genes

Young leaves were collected from plants after pathotyping with FORL. Plant genomic DNA was then isolated using the Wizard Magnetic Kit (Promega) following the manufacturer’s instructions. DNA was extracted separately from each individual of the F₂ plant lines. The resistant and susceptible bulks were generated from twenty resistant and twenty susceptible F₂ individuals, respectively, as described (Devran et al. 2015 (link)) and used for genomic sequencing. We generated 1 lane of 100 bp paired-end Illumina HiSeq 2500 sequencing data for each parent (resistant and susceptible) line and bulked (resistant and susceptible) pools.

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Devran Z., Kahveci E., Hong Y., Studholme D.J, & Tör M. (2018). Identifying molecular markers suitable for Frl selection in tomato breeding. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(10), 2099-2105.

Publication 2018

Wizard Magnetic Kit HiSeq 2500

Bp 100 Genomic Parent Plant Promega

Corresponding Organization :

Other organizations : Akdeniz University, Hangzhou Normal University, University of Exeter, University of Worcester

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Variable analysis

independent variables

Pathotyping with FORL
Resistant and susceptible F2 plant lines

dependent variables

Plant genomic DNA isolated from young leaves
Resistant and susceptible bulks generated from F2 individuals
Genomic sequencing of parent lines and bulked pools

control variables

Plant genomic DNA isolation using Wizard Magnetic Kit (Promega)
DNA extraction from individual F2 plants

controls

Positive control: Not mentioned
Negative control: Not mentioned

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