Protocol detail

Luci-eASCs Characterization Criteria

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Luci-eASCs were defined according to the criteria of the International Society for Cellular Therapy [36 (link)]. Luci-eASCs were stained using antibodies for CD73 (AD2), CD90 (5E10, both from Becton Dickinson), CD105 (43A3, Biolegend, San Diego, CA, USA), CD14 (RM052, Immunotech, Monrovia, CA, USA), CD34 (8G12, Becton Dickinson, Franklin Lakes, NJ, USA), CD45 (J33, Beckman Coulter, Brea, CA, USA) and HLA-DR (AF6-120.1, eBiosciences, Waltham, MA, USA) [18 (link)].

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Lopez-Santalla M., Mancheño-Corvo P., Escolano A., Menta R., Delarosa O., Redondo J.M., Bueren J.A., Dalemans W., Lombardo E, & Garin M.I. (2018). Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis. International Journal of Molecular Sciences, 19(7), 1853.

Publication 2018

CD105 (43A3, CD34 (8G12, CD45 (J33, AF6-120.1,

Antibodies Cd73 Cd90 Cellular therapy Hla dr

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Variable analysis

independent variables

Luci-eASCs (defined according to the criteria of the International Society for Cellular Therapy)

dependent variables

Expression of cell surface markers: CD73, CD90, CD105, CD14, CD34, CD45, and HLA-DR

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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