A trained phlebotomist will extract, treat and subsequently store the blood at approximately -80°C until later analysis. Analysis of blood samples through commercial ELISA kits (Thermo Fisher Scientific Australia Pty Ltd., Victoria, Australia; Randox Laboratories Ltd., West Virginia, USA; R&D Systems Inc., Minneapolis, USA) will be used for PSA, IGF-1, IGF-2, IGFBP3, IL-6, IL-8, Hepcidin, total cholesterol, and triglycerides analysis.
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