Genome-wide DNA Methylation Analysis

500 ng of genomic DNA from each sample was treated with sodium bisulfite in duplicate, using the EZ96 DNA methylation kit (Zymo Research, CA, USA) following the manufacturer’s standard protocol. Genome-wide DNA methylation was assessed using the Illumina Infinium HumanMethylation450 BeadChip (Illumina Inc, CA, USA) according to manufacturer’s instructions. Illumina GenomeStudio software was used to extract the raw signal intensities of each probe (without background correction or normalization).

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Pidsley R., Y Wong C.C., Volta M., Lunnon K., Mill J, & Schalkwyk L.C. (2013). A data-driven approach to preprocessing Illumina 450K methylation array data. BMC Genomics, 14, 293.

Publication 2013

Dna methylation Genome Sodium bisulfite

Corresponding Organization :

Other organizations : King's College London, University of Exeter

Top 5 similar protocols

Protocol cited in 347 other protocols

Variable analysis

independent variables

Sodium bisulfite treatment

dependent variables

Genome-wide DNA methylation

control variables

Genomic DNA amount (500 ng) per sample
Duplicate treatment with sodium bisulfite
Use of EZ96 DNA methylation kit (Zymo Research, CA, USA) following manufacturer's standard protocol
Genome-wide DNA methylation assessment using Illumina Infinium HumanMethylation450 BeadChip (Illumina Inc, CA, USA) following manufacturer's instructions
Illumina GenomeStudio software used to extract raw signal intensities of each probe (without background correction or normalization)

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