Immunohistochemistry and Western Blot Analysis

Immunohistochemistry (IHC) experiments and IHC scoring were carried out as previously described according to previous protocols [25 (link)]. Protein lysates were prepared by homogenization in 1% NP-40 containing 1 mM PMSF, and samples containing 20 μg of protein were separated by SDS-PAGE. The immunoreactive bands were visualized using an ImmobilonTM Western kit (Millipore, Billerica, MA) using the Syngene G:BOX imaging system (Frederick, USA). According to the manufacturer’s instructions, western blot assays were carried out with antibodies diluted in PBS plus Tween-20 (PBST). Information on all primary antibodies used is listed in Supplementary Table 2.