Whole-cell protein lysates were prepared, separated on SDS polyacrylamide gel and transferred to nitrocellulose membrane according to a previously described protocol [36 (link)]. After primary antibody incubation, membranes were incubated with IRD680- and IRD800-conjugated secondary antibodies (LI-COR), and scanned on a LICOR Odyssey CLx imaging system. Antibodies against FLAG and β-tubulin were purchased from Sigma-Aldrich. Quantitative analyses were performed using NIH Image J.
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