Preparation of 15d-PGJ2-Loaded PLGA Nanoparticles

The 15d-PGJ2-NP production was prepared according to the protocol established by Alves et al. [11 (link)]. The PLGA Polymer (100 mg) was dissolved into 30 mL acetone with 15d-PGJ2 (100 μg; Sigma-Aldrich, St. Louis, MO, USA), sorbitan monostearate (40 mg), and caprylic/capric acid triglyceride (200 mg) the organic phase. Then, polysorbate 80 (60 mg) and deionized water (30 mL) were added, comprising the aqueous phase. Briefly, after the components in both phases were dissolved, the organic phase was gently added to the aqueous phase, and the suspension was maintained under agitation for 10 min. The solvent acetone (60/40 v/v). was removed under evaporation, and the suspension was concentrated to a volume of 10 mL under low pressure, using a rotary evaporator, to obtain a suspension of 15d-PGJ2 with a final concentration of 10 μg/mL. Residual solvent was removed by evaporation until there were no observed traces of acetone in the preparation and analyzed by HPLC, as previously described (Napimoga et al., 2012) [9 (link)].