Genetically Modified Mouse Models

Thbs1-deficient (Thbs1^-/- (#006141)), LysM-Cre (#004781) and Rosa26-EYFP (#006148) mice were purchased from Jackson Laboratories. C57BL/6 mice, GFP transgenic mice (C57BL/6-Tg (CAG-EGFP)) mice were purchased from Japan SLC,Inc. Systemic CD47-deficient (CD47^-/-) (CD47^f/f;E2A-Cre) mice were generated by crossing CD47^f/f mice with E2A-Cre mice^{49 (link)}. Thbs1^f/f mice were generated as previously described^{39 (link)}. CD36-deficient (CD36^-/-) mice were kindly provided by Dr. Freeman (Harvard Medical School). All mouse strains were generated in a C57BL/6 background and were born and maintained under pathogen-free conditions. These mice were maintained in 14 hr light/10 h dark cycle, and the housing temperature and humidity were maintained were 24 °C and 50%, respectively. Animal handling and experimental procedures conformed to institutional guidelines and were approved by the animal research committee of Kyoto University (Kyoto, Japan) and performed in accordance with Japanese government regulations. 8–10 weeks old male mice were used in all experiments.