AOPP Quantification Protocol

Determination of AOPP levels was performed by modification of the method of Witko-Sarsat [23 (link)]. Two mL of plasma was diluted 1 : 5 in PBS: 0.1 mL of 1.16 M potassium iodide was then added to each tube, followed by 0.2 mL acetic acid after 2 min. The absorbance of the reaction mixture was immediately read at 340 nm against a blank containing 2 mL of PBS, 0.1 mL of KI, and 0.2 mL of acetic acid. The chloramine-T absorbance at 340 nm being linear within the range of 0 to 100 mmol/L, AOPP concentrations were expressed as μmol·L⁻¹ chloramine-T equivalents.

Free full text: Click here

Tiwari B.K., Kumar D., Abidi A.B, & Rizvi S.I. (2014). Efficacy of Composite Extract from Leaves and Fruits of Medicinal Plants Used in Traditional Diabetic Therapy against Oxidative Stress in Alloxan-Induced Diabetic Rats. ISRN Pharmacology, 2014, 608590.

Publication 2014

Acetic acid Aopp Chloramine t Plasma

Corresponding Organization :

Other organizations : Sam Higginbottom Institute of Agriculture, University of Allahabad

Top 5 similar protocols

Protocol cited in 13 other protocols

Variable analysis

dependent variables

AOPP levels

control variables

Plasma dilution ratio (1 : 5 in PBS)
Volume of potassium iodide (0.1 mL of 1.16 M)
Volume of acetic acid (0.2 mL)
Reaction time (2 min)
Absorbance measurement (340 nm)
Blank (2 mL of PBS, 0.1 mL of KI, and 0.2 mL of acetic acid)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!