Epigenetic Regulation via MBD2 Silencing

For the CAF-CM treatment, cells were seeded at 1.10⁵ per well in six-well plates. The following day, cells were cultured in CAF-CM for 48 h. For MBD2 siRNA experiments, cells were seeded at 3.10⁵ per well in six-well plates. The day after, cells were transfected with 100 pmol of MBD2-targeting siRNA (siMBD2; sense: 5′-GGAGGAAGUGAUCCGAAAdTdT-3′)^{39 (link)} or control siRNA (Sigma-Aldrich, MISSION siRNA Universal Negative Controls #1) using RNAiMAX (Sigma-Aldrich, Saint-Quentin-Fallavier, France) as specified by the manufacturer’s instructions. Cells were collected 72 h after treatment initiation. For the DAC treatment, cells were seeded at 1.10⁵ per well in six-well plates. The following day, cells were treated daily with 10 μM of DAC (Sigma-Aldrich) for 3 days and were then collected.