Sequencing analysis of positive scFv clones of the soluble phage ELISA was performed as described previously (43 (link), 44 (link)). Briefly, the plasmid of positive scFv clones from the soluble phage ELISA was isolated by using plasmid purification Mini kit (Qiagen, Germany) and sequenced commercially by Macrogen (South Korea). The sequences were analyzed using IMGT V Quest software.
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