Western Blot Protein Detection

Samples were prepared as described previously^{75 (link)}. Anti BLIMP1 (#9115), CCND2 (#3741S), S100A4 (#13018S), S100A6 (#13162S) Abs were purchased from Cell Signaling (New England Biolabs, Frankfurt, Germany). Anti GAPDH (ab8245) and anti S100A2 (ab 109494) Abs were from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).

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Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A., Nagel S., Steube K., Uphoff C.C, & Drexler H.G. (2019). The LL-100 panel: 100 cell lines for blood cancer studies. Scientific Reports, 9, 8218.

Publication 2019

Anti GAPDH (ab8245) biotin/streptavidin-horseradish peroxidase system Renaissance Western Blot Chemoluminescence Reagent”

Biotin Blimp1 Ccnd2 Gapdh Horseradish peroxidase Membranes Nitrocellulose Streptavidin Western blot

Corresponding Organization : Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures

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Variable analysis

independent variables

Anti BLIMP1 (#9115)
CCND2 (#3741S)
S100A4 (#13018S)
S100A6 (#13162S)

dependent variables

Specific bands on nitrocellulose membranes

control variables

GAPDH (ab8245)
S100A2 (ab 109494)

controls

Positive control: Biotin/streptavidin-horseradish peroxidase system
Negative control: Not specified

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