Samples were prepared as described previously75 (link). Anti BLIMP1 (#9115), CCND2 (#3741S), S100A4 (#13018S), S100A6 (#13162S) Abs were purchased from Cell Signaling (New England Biolabs, Frankfurt, Germany). Anti GAPDH (ab8245) and anti S100A2 (ab 109494) Abs were from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).
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