Western Blot Analysis of Testis Proteins

The BmN4 cell lysates were subjected to western blots as described previously^{15 (link),34 (link)}. The following antibodies were used: 17.8 anti-Mili (1:1000)^{56 (link)}, BMK5263 anti-BmRNase κ (1:500), S213 anti-Siwi (1:1000)^{15 (link)}, anti-BmAgo3 (1:500)^{29 (link)}, anti-BmPapi (1:1000)^{11 (link)}, BmVasa571 anti-BmVasa (1:500)^{34 (link),60}, anti-Hsp60 (Cell Signaling Technology, #4870, 1:2000), anti-Tom20 (Santa Cruz Biotechnology, SC-11415, 1:1000), anti-β-Actin (Abcam, ab8224, 1:2000), anti-β-Tubulin (Developmental Studies Hybridoma Bank, E7, 1:2000), and anti-FLAG (Sigma, F3165, 1:1000).

Free full text: Click here

Shigematsu M., Kawamura T., Morichika K., Izumi N., Kiuchi T., Honda S., Pliatsika V., Matsubara R., Rigoutsos I., Katsuma S., Tomari Y, & Kirino Y. (2021). RNase κ promotes robust piRNA production by generating 2′,3′-cyclic phosphate-containing precursors. Nature Communications, 12, 4498.

Publication 2021

anti-Hsp60 anti-Tom20 anti-β-Actin anti-β-Tubulin anti-FLAG

Actin Antibodies Cell Hybridoma Tubulin Western blots

Corresponding Organization :

Other organizations : Thomas Jefferson University, The University of Tokyo

Top 5 similar protocols

Variable analysis

independent variables

BmN4 cell lysates

dependent variables

Protein expression levels of Mili, BmRNase κ, Siwi, BmAgo3, BmPapi, BmVasa, Hsp60, Tom20, β-Actin, and β-Tubulin

control variables

Western blot protocol described previously
Antibody concentrations used: 17.8 anti-Mili (1:1000), BMK5263 anti-BmRNase κ (1:500), S213 anti-Siwi (1:1000), anti-BmAgo3 (1:500), anti-BmPapi (1:1000), BmVasa571 anti-BmVasa (1:500), anti-Hsp60 (1:2000), anti-Tom20 (1:1000), anti-β-Actin (1:2000), anti-β-Tubulin (1:2000), and anti-FLAG (1:1000)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!