We replicated the identified novel loci in two independent data sets for which genotype data were available: the European Prospective Investigation into Cancer and Nutrition (EPIC) study with 40,562 individuals across two nested case-control studies (EPIC-InterAct and EPIC-CVD) and the cohort-wide EPIC-Norfolk study (Supplementary Note 1); and a cohort of 2195 individuals (all controls) additionally collected as part of the SOCCS that were not included in our discovery stage. As for the phenotype, EPIC individuals were assayed for plasma 25-hydroxyvitamin D3 and SOCCS individuals were assayed for total 25-hydroxyvitamin D. We performed the association analysis in a similar manner, adjusted for age, sex, time of sample collection, and study center where relevant. We regarded P-value < 0.05 in the replication samples, and P-value < 5×10−8 in the pooled analysis as successful replication.
Jiang X., O’Reilly P.F., Aschard H., Hsu Y.H., Richards J.B., Dupuis J., Ingelsson E., Karasik D., Pilz S., Berry D., Kestenbaum B., Zheng J., Luan J., Sofianopoulou E., Streeten E.A., Albanes D., Lutsey P.L., Yao L., Tang W., Econs M.J., Wallaschofski H., Völzke H., Zhou A., Power C., McCarthy M.I., Michos E.D., Boerwinkle E., Weinstein S.J., Freedman N.D., Huang W.Y., Van Schoor N.M., van der Velde N., Groot L.C., Enneman A., Cupples L.A., Booth S.L., Vasan R.S., Liu C.T., Zhou Y., Ripatti S., Ohlsson C., Vandenput L., Lorentzon M., Eriksson J.G., Shea M.K., Houston D.K., Kritchevsky S.B., Liu Y., Lohman K.K., Ferrucci L., Peacock M., Gieger C., Beekman M., Slagboom E., Deelen J., Heemst D.V., Kleber M.E., März W., de Boer I.H., Wood A.C., Rotter J.I., Rich S.S., Robinson-Cohen C., den Heijer M., Jarvelin M.R., Cavadino A., Joshi P.K., Wilson J.F., Hayward C., Lind L., Michaëlsson K., Trompet S., Zillikens M.C., Uitterlinden A.G., Rivadeneira F., Broer L., Zgaga L., Campbell H., Theodoratou E., Farrington S.M., Timofeeva M., Dunlop M.G., Valdes A.M., Tikkanen E., Lehtimäki T., Lyytikäinen L.P., Kähönen M., Raitakari O.T., Mikkilä V., Ikram M.A., Sattar N., Jukema J.W., Wareham N.J., Langenberg C., Forouhi N.G., Gundersen T.E., Khaw K.T., Butterworth A.S., Danesh J., Spector T., Wang T.J., Hyppönen E., Kraft P, & Kiel D.P. (2018). Genome-wide association study in 79,366 European-ancestry individuals informs the genetic architecture of 25-hydroxyvitamin D levels. Nature Communications, 9, 260.
Publication 2018
25 hydroxyvitamin d Cancer EuropeanGenotype Nutrition investigationPhenotypePlasma Replication Sample collection
Corresponding Organization :
Other organizations :
Harvard University, King's College London, Hebrew SeniorLife, Boston University, Stanford University, Medical University of Graz, University College London, University of Cambridge, MRC Epidemiology Unit, University of Maryland, Baltimore, National Cancer Institute, University of Minnesota, Indiana University – Purdue University Indianapolis, Universitätsmedizin Greifswald, German Centre for Cardiovascular Research, University of South Australia, Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, University of Oxford, Johns Hopkins University, The University of Texas Health Science Center at Houston, Amsterdam UMC Location VUmc, Erasmus MC, Erasmus University Rotterdam, Wageningen University & Research, Framingham Heart Study, Jean Mayer Human Nutrition Research Center on Aging, Tufts University, University of Helsinki, University of Gothenburg, Sahlgrenska University Hospital, Helsinki University Hospital, Wake Forest University, National Institute on Aging, Helmholtz Zentrum München, Leiden University Medical Center, Heidelberg University, University Hospital Heidelberg, University Medical Centre Mannheim, University of Washington, Children's Nutrition Research Center at Baylor College of Medicine, Harbor–UCLA Medical Center, The Lundquist Institute, University of Virginia, Vanderbilt University Medical Center, Imperial College London, University of Edinburgh, MRC Institute of Genetics and Molecular Medicine, Western General Hospital, Uppsala University, Trinity College Dublin, Institute for Molecular Medicine Finland, Finland University, Fimlab (Finland), Tampere University, Tampere University Hospital, Turku University Hospital, Research Council of Finland, Vitas (Norway), Wellcome Sanger Institute, Vanderbilt University, Massachusetts Institute of Technology, Broad Institute
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