The thiobarbituric acid-reactive species (TBARS) assay was used to assess 100 μL of supernatant of homogenized left cerebral hemisphere. This method followed Laoung-on et al. [11 (link)], and then, the mixture solution was measured by a microplate reader at 532 nm (Bio Tek Synergy H4 Hybrid Microplate Reader, BioTek Instruments, Winooski, VT, USA).
Free full text: Click here