Human HeLa and 293-IL-17RA stable cells were treated with 2 μM AZD5363 (Selleck Chemicals, Inc.), 50 ng/ml insulin (Sigma Aldrich), and/or 20 ng/ml recombinant human IL-17A (R & D Systems) for 2 h. Mouse embryonic fibroblasts (MEFs) with wild-type Gsk3β+/+ or knockout Gsk3β−/− were treated with 20 ng/ml recombinant mouse IL-17A, IL-17F, IL-17C, or IL-17E (R & D Systems) for 2 h. RNA was isolated for qRT-PCR analysis as described previously [33 (link)].
Liu S., Zhang Q., Chen C., Ge D., Qu Y., Chen R., Fan Y.M., Li N., Tang W.W., Zhang W., Zhang K., Wang A.R., Rowan B.G., Hill S.M., Sartor O., Abdel A.B., Myers L., Lin Q, & You Z. (2016). Hyperinsulinemia enhances interleukin-17-induced inflammation to promote prostate cancer development in obese mice through inhibiting glycogen synthase kinase 3-mediated phosphorylation and degradation of interleukin-17 receptor. Oncotarget, 7(12), 13651-13666.
Azd5363Cells EmbryonicFibroblasts Gsk3β Hela Human Human il 17a Il 17a Il 17c Il 17e Il 17f Il 17raInsulin Mouse
Corresponding Organization : Louisiana Cancer Research Center
Other organizations :
Guangdong Medical College, Ochsner Medical Center, Xavier University of Louisiana, University at Albany, State University of New York
Cell types: Human HeLa cells, 293-IL-17RA stable cells, Mouse embryonic fibroblasts (MEFs) with wild-type Gsk3β+/+ or knockout Gsk3β−/−
Treatment duration: 2 hours
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
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