Luciferase Assay for Zebrafish Larvae
Corresponding Organization :
Other organizations : Universidad de Murcia, Hospital Universitario Virgen de la Arrixaca, Centro de Investigación Biomédica en Red
Variable analysis
- Microinjection of 200 pg of in vitro-transcribed zfiGLuc or GLuc RNA into embryos
- Luciferase signal measured on a Luminometer Optocomp II
- Embryos were microinjected
- Larvae were infected with WT ST (MOI of 10)
- Larval extracts were obtained at 24 hpi
- Larval extracts were combined 1:1 with distilled water containing 4.4 μM coelenterazine
- Positive control: Not specified
- Negative control: Not specified
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