Embryos were microinjected as described above with 200 pg of in vitro-transcribed zfiGLuc or GLuc RNA. After 48 h, larvae were infected with WT ST (MOI of 10) and whole-larval extracts were obtained at 24 hpi as described previously65 (link). Larval extracts were then combined 1:1 with distilled water containing 4.4 μM coelenterazine (Sigma-Aldrich) to achieve a final concentration of 2.2 μM. The luciferase signal was then measured on a Luminometer Optocomp II (MGM Instruments).
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