Annexin V-PE Assay for T Cell Apoptosis

Flow cytometry assays were performed for T cell apoptosis determination using the Annexin V-PE kit (BD Biosciences, San Jose, CA, USA) as previously described [28 (link)]. In brief, freshly sorted T cells were cultured in the presence of tested doses of rHcADRM1 proteins (0, 5, 10, 20 and 40 µg/ml) followed by Annexin V and 7-aminoactinomycin D (7-AAD) staining based on the kit’s specification. The PBS-stimulated T cells served as negative controls. Three individual tests, each in triplicate, were conducted.

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Lu M., Tian X., Zhang Y., Aimulajiang K., Wang W., Ehsan M., Li C., Yan R., Xu L., Song X, & Li X. (2020). Unveiling the immunomodulatory properties of Haemonchus contortus adhesion regulating molecule 1 interacting with goat T cells. Parasites & Vectors, 13, 424.

Publication 2020

Annexin V-PE kit

7 aad Annexin v Apoptosis Assays Flow cytometry Proteins T cells

Corresponding Organization : Nanjing Agricultural University

Other organizations : Beltsville Agricultural Research Center, Agricultural Research Service

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Variable analysis

independent variables

Tested doses of rHcADRM1 proteins (0, 5, 10, 20 and 40 µg/ml)

dependent variables

T cell apoptosis

control variables

Freshly sorted T cells

controls

Negative control: PBS-stimulated T cells

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