Elemental Composition of Rice Husk Extract

For determination of the elemental composition of the rice husk extract (RHE), milling and subsequent screening of the husk were performed, while to obtain the solids contained in the rice husk extract, lyophilization was used (Labconco company, Kansas City, MO, USA). The resulting solid was weighed (0.2 g) and analyzed by X-ray fluorescence (Panalytical, Epsilon 1, Almelo, The Netherlands), before being integrated by a spectrometer with the use of the Omniam software [35 (link)]. For the total solids content in the rice husk extract, the liquid part was evaporated, and the rest of the solids were weighed and heated at 600 °C for 3 h for the de-ashed content.