For electrophysiology experiments, the tissue block containing the injection site was stored overnight in 10% neutral-buffered formalin at 4°C. After, it was transferred into 0.2% PBS Azide at 4°C until sectioning. The tissue block was mounted onto a stage and sectioned in 0.1M PBS at a thickness of 100μm using a VT-1000 vibratome (Leica). Slices were mounted onto microscope slides using DAPI Fluoromount-G (Southern Biotech #0100–20) and coverslipped before confocal imaging.
Following all behavioral experiments, mice were anesthetized with an intraperitoneal injection of Ketamine-Xylazine (120mg/kg Ketamine, 24 mg/kg Xylazine) and transcardially perfused with PBS followed by 10% neutral-buffered formalin. The brain was delicately extricated and stored in 10% neutral-buffered formalin overnight at 4°C. Tissue was mounted onto a stage and sectioned at 100μm using a VT-1000 vibratome (Leica). Sections were mounted and coverslipped using DAPI Fluoromount-G (Southern Biotech #0100–20). Confocal images were acquired using a LSM800 confocal laser scanning microscope (Zeiss) for injection site location verification, cannula placement, and viral expression in POm axons within pDLS and stereotypical POm-cortical projections in S1 L1 and L5a of all experimental mice.4 (link),25 (link),26 (link),47 (link),49 (link) All data were acquired using the Zen software suite (Zeiss).
Following all behavioral experiments, mice were anesthetized with an intraperitoneal injection of Ketamine-Xylazine (120mg/kg Ketamine, 24 mg/kg Xylazine) and transcardially perfused with PBS followed by 10% neutral-buffered formalin. The brain was delicately extricated and stored in 10% neutral-buffered formalin overnight at 4°C. Tissue was mounted onto a stage and sectioned at 100μm using a VT-1000 vibratome (Leica). Sections were mounted and coverslipped using DAPI Fluoromount-G (Southern Biotech #0100–20). Confocal images were acquired using a LSM800 confocal laser scanning microscope (Zeiss) for injection site location verification, cannula placement, and viral expression in POm axons within pDLS and stereotypical POm-cortical projections in S1 L1 and L5a of all experimental mice.4 (link),25 (link),26 (link),47 (link),49 (link) All data were acquired using the Zen software suite (Zeiss).
