Western Blot Analysis of COX-2 Expression

Western blotting was performed as described previously (Lai et al., 2019 (link)) The primary antibodies used in this study were anti-COX-2 (SC-1745; 1:500; Santa Cruz Biotechnology, Dallas, USA). and anti-ß-actin (A5441; 1:5000; Sigma Aldrich, St. Loius, USA). Bound antibodies were detected with peroxidase conjugated secondary antibodies and enhanced chemiluminescence reagents (Advansta, Menlo Park, CA, USA). ImageJ was used to measure signal intensities.

Free full text: Click here

Abdul Rahman M., Tan M.L., Johnson S.P., Hollows R.J., Chai W.L., Mansell J.P., Yap L.F, & Paterson I.C. (2020). Deregulation of lysophosphatidic acid metabolism in oral cancer promotes cell migration via the up-regulation of COX-2. PeerJ, 8, e10328.

Publication 2020

anti-COX-2 (SC-1745 anti-ß-actin (A5441 enhanced chemiluminescence reagents

Actin Antibodies Chemiluminescence Cox 2 Peroxidase

Corresponding Organization :

Other organizations : University of Malaya, National University of Malaysia, Northern Devon Healthcare NHS Trust, University of Birmingham, University of the West of England

Top 5 similar protocols

Variable analysis

independent variables

Primary antibodies used: anti-COX-2 (SC-1745; 1:500; Santa Cruz Biotechnology, Dallas, USA) and anti-ß-actin (A5441; 1:5000; Sigma Aldrich, St. Loius, USA)

dependent variables

Signal intensities measured using ImageJ

control variables

Western blotting protocol as described previously (Lai et al., 2019)
Bound antibodies detected with peroxidase conjugated secondary antibodies and enhanced chemiluminescence reagents (Advansta, Menlo Park, CA, USA)

controls

No specific positive or negative controls were mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!