Protocol detail

Antibody Characterization for STING

Find Similar Protocols

Rabbit polyclonal antibody against a synthetic peptide corresponding to residues 324–340 of human STING was obtained from EvoQuest Custom Antibody Services (Invitrogen, CA). Other antibodies were obtained from following sources: SEC61β (Upstate, NY), β-Actin, HA, FLAG (Sigma, MO), COX IV, Calreticulin, IRF3 and RIG-I (Abcam, MA).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Ishikawa H, & Barber G.N. (2008). STING an Endoplasmic Reticulum Adaptor that Facilitates Innate Immune Signaling. Nature, 455(7213), 674-678.

Publication 2008

Actin Antibodies Antibody Calreticulin Human Irf3 Peptide Rabbit Rig i

Top 5 similar protocols

Protocol cited in 8 other protocols

Variable analysis

independent variables

Rabbit polyclonal antibody against a synthetic peptide corresponding to residues 324–340 of human STING

dependent variables

Not explicitly mentioned

control variables

SEC61β (Upstate, NY)
β-Actin (Sigma, MO)
HA (Sigma, MO)
FLAG (Sigma, MO)
COX IV (Abcam, MA)
Calreticulin (Abcam, MA)
IRF3 (Abcam, MA)
RIG-I (Abcam, MA)

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!