Western Blot Analysis of Fpn1, Stat3, and Smad1/5/9

Protein extraction and Western blotting were performed as described previously (Dichtl et al., 2019 (link)). The antibodies used were a rabbit Fpn1 antibody (1:2000; Eurogentec), a rabbit actin antibody (1:500; Sigma Aldrich), a rabbit Stat3 antibody (1:2000, Cell Signaling), a rabbit phospho-Stat3 antibody (1:2000, Cell Signaling), a rabbit phospho-Smad1/5/9 antibody (1:2000, Cell Signaling) and appropriate HRP-conjugated secondary antibodies (1:2000, anti-rabbit; Dako). For quantification, densitometry data were acquired on a ChemiDoc Touch Imaging System (Bio-Rad Laboratories GmbH) and analyzed with Quantity One software (Bio-Rad Laboratories GmbH).

Free full text: Click here

Hoffmann A., de Souza L.V., Seifert M., von Raffay L., Haschka D., Grubwieser P., Grander M., Mitterstiller A.M., Nairz M., Poli M, & Weiss G. (2021). Pharmacological Targeting of BMP6-SMAD Mediated Hepcidin Expression Does Not Improve the Outcome of Systemic Infections With Intra-Or Extracellular Gram-Negative Bacteria in Mice. Frontiers in Cellular and Infection Microbiology, 11, 705087.

Publication 2021

rabbit Fpn1 antibody rabbit phospho-Smad1/5/9 antibody ChemiDoc Touch Imaging System Quantity One software

Actin Antibodies Antibody Densitometry Protein Rabbit Stat3 Touch

Corresponding Organization : Innsbruck Medical University

Other organizations : University of Brescia

Top 5 similar protocols

Variable analysis

independent variables

Protein extraction and Western blotting protocol

dependent variables

Fpn1 protein expression
Actin protein expression
Stat3 protein expression
Phospho-Stat3 protein expression
Phospho-Smad1/5/9 protein expression

control variables

Appropriate HRP-conjugated secondary antibodies (1:2000, anti-rabbit; Dako)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!