Generation of DsRed OT-I and PLVAP iLECKO Mice

Specific pathogen-free C57BL/6, B6(Cg)-Tyrc-2J/J, and Ifnar^–/– mice were obtained from Taconic Farms. STOCK Tg (CAG-DsRed*MST) 1Nagy/J mice were crossed to OT-I TCR transgenic mice (NIAID Intramural Research Repository) and bred for homozygosity to create DsRed OT-I mice. Plvap^fl/fl mice ^{29 (link)} on C57BL/6J background were crossed to Prox1-CreERT2^tg/+ mice ^{51 (link)} to create PLVAP^iLECKO mice (genotype: Plvap^fl/flProx1-CreERT2^tg/+). Prox1-CreERT2^tg/+ mice on C57BL/6 genetic background were a kind gift from G. Oliver (U Chicago). Plvap^iLECKO and littermate Plvap^fl/fl control mice were bred in house at Dartmouth and shipped to NIH. 6- to 16-week-old female mice were used in all experiments unless otherwise noted. Mice were housed under specific pathogen-free conditions (including murine norovirus, mouse parvovirus, and mouse hepatitis virus) and maintained on standard rodent chow and water supplied ad libitum. All animal studies were approved by and performed in accordance with the Animal Care and Use Committee of the National Institute of Allergy and Infectious Diseases.

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Reynoso G.V., Weisberg A.S., Shannon J.P., McManus D.T., Shores L., Americo J.L., Stan R.V., Yewdell J.W, & Hickman H.D. (2019). Lymph Node Conduits Transport Virions for Rapid T Cell Activation. Nature immunology, 20(5), 602-612.

Publication 2019

C57BL/6 B6(Cg)-Tyrc-2J/J Ifnar–/–

Animal C57bl mice Female Genetic background Genotype Homozygosity Mouse Mouse hepatitis virus Murine Norovirus Parvovirus Rodent Specific pathogen free Transgenic mice

Corresponding Organization :

Other organizations : National Institute of Allergy and Infectious Diseases, National Institutes of Health, Dartmouth College

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Variable analysis

independent variables

Mouse strains: C57BL/6, B6(Cg)-Tyrc-2J/J, Ifnar–/–, DsRed OT-I, and PLVAP iLECKO
Genotypes: Plvapfl/fl, Prox1-CreERT2tg/+, and their combination PLVAP iLECKO

dependent variables

Not explicitly mentioned

control variables

Age of mice: 6- to 16-week-old female mice
Housing conditions: Specific pathogen-free, maintained on standard rodent chow and water ad libitum

controls

Positive control: Plvapfl/fl mice
Negative control: Littermate Plvapfl/fl control mice

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