SNP Genotyping from Paraffin Tissues

Genomic deoxyribonucleic acid was extracted from paraffin embedded tissue using GeneJET Genomic deoxyribonucleic acid Purification Kit (ThermoScientific), according to manufacturer protocol. SNPs polymorphisms of the TCF7L2 (rs7903146, C/T), cancer susceptibility 21 (rs6983267, G/T), and GREM1 (rs1696981, C/T) were identified using a real-time PCR method based on the TaqMan® Genotyping Master Mix (Applied Biosystems) and 20× SNP Genotyping Assay (Applied Biosystems), using a 7500 Fast Real-Time Systems (Applied Biosystems) according to manufacturer procedure. Allelic discrimination was made with the help of 7500 Fast Real-Time PCR software, version 2.3.

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Mitroi A.F., Leopa N., Dumitru E., Dumitru A., Tocia C., Popescu I., Mitroi A, & Popescu R.C. (2023). TCF7L2, CASC8, and GREM1 polymorphism and colorectal cancer in south-eastern Romanian population. Medicine, 102(7), e33056.

Publication 2023

TaqMan® Genotyping Master Mix 20× SNP Genotyping Assay 7500 Fast Real-Time Systems

Allelic Assay Cancer Deoxyribonucleic acid Discrimination Embedded paraffin Genomic Grem1 Polymorphisms Real time pcr Susceptibility Tcf7l2 Tissue

Corresponding Organization : Ovidius University

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Variable analysis

independent variables

Rs7903146 (C/T) in TCF7L2
Rs6983267 (G/T) in cancer susceptibility 21
Rs1696981 (C/T) in GREM1

dependent variables

SNPs polymorphisms

control variables

Genomic deoxyribonucleic acid extracted from paraffin embedded tissue
GeneJET Genomic deoxyribonucleic acid Purification Kit (ThermoScientific)
TaqMan® Genotyping Master Mix (Applied Biosystems)
20× SNP Genotyping Assay (Applied Biosystems)
7500 Fast Real-Time Systems (Applied Biosystems)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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